dPIP5K is not required to support cytoskeleton function and dynamin mediated endocytosis in adult photoreceptors.

<p>(A) Confocal images of phalloidin stained retinae from control and <i>dPIP5K¹⁸</i> photoreceptors showing normal staining of the rhabdomeres. (B) Confocal images of retinae stained with a p-moesin specific antibody from control and <i>dPIP5K¹⁸</i>. Red pseudo color represents p-moesin staining and green marks the rhabdomeric region stained with phalloidin. (C) Confocal images showing longitudinal sections from retinae stained with an antibody to Rh1. The arrows indicated Rhodopsin Loaded Vesicle (RLV) involved in Rh1 endocytosis and recycling. (D) Rate of photoreceptor degeneration in <i>norpA^P24</i> and <i>norpA^P24; dPIP5K¹⁸</i> monitored using optical neutralization. The flies were reared in continuous light at 2300 lux. The X-axis represents age of the flies and the Y-axis represents number of rhabdomere visualized in each ommatidium. Error bars represent mean +/− S.D from 50 ommatidia taken from at least five flies. (E) Representative transmission electron micrographs of retinae from <i>norpA^P24</i> and <i>norpA^P24; dPIP5K¹⁸</i> flies showing the degree of preservation of ultrastructure. Images shown are from flies that are three days old gown under the same illumination conditions as for panel D. (F) Quantitative representation of Rhodopsin Loaded Vesicles (RLVs) in control, <i>norpA^p24</i> and <i>norpA^p24;dPIP5K¹⁸</i>. The genotype of the fly is shown in the X-axis and Y-axis represents the count of RLVs.