IFN and empty plasmid transfection induce PLSCR1 mRNA and protein.

<p>(<b>A</b>) T80 cells were treated with 3000 IU/ml IFN-2α from 15 minutes up to 24 hours. Cell lysates were analyzed by western blotting with the indicated antibodies (n = 3). (<b>B</b>) Total RNA was isolated from cells treated as described in (A). PLSCR1 mRNA levels, detected via real-time PCR, are presented (n = 3). (<b>C</b>) T80 cells were transfected with empty pcDNA3 plasmid (“pcDNA3”) or transfection reagent only (“mock”). Cell lysates were harvested from 6 up to 48 hours post-transfection and analyzed via western blotting with the indicated antibodies (n = 3). (<b>D</b>) Total RNA was isolated from cells treated as described in (<b>C</b>). PLSCR1 mRNA levels, detected via real-time PCR, are presented (n = 4). (<b>E</b>) HMEC cells were treated similarly as described for T80 cells (C) and cell lysates were then analyzed by western blotting with the indicated antibodies (n = 2). (<b>F</b>) Annexin V-PI staining was performed in 48 hours mock or pcDNA3 transfected cells (n = 3).