RanBPM N-terminal region contains several determinants that regulate its subcellular localization.

<p><b>A)</b> Schematic diagram of N-terminal mutant RanBPM constructs. <b>B)</b> Mutant RanBPM (ΔN1-4) were transfected and scored as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0117655#pone.0117655.g001" target="_blank">Fig. 1</a>. Data represent averages from three separate experiments, each assessing a minimum of 100 cells. Error bars represent SD. Mutant RanBPM constructs versus WT, ***, <i>P</i><0.001; **, <i>P</i><0.01; *, <i>P</i><0.05. <b>C)</b> Representative images of transfected mutant RanBPM localization quantified in B. <b>D)</b> Identification of a nuclear targeting sequence in the extreme N-terminal region. Mutants with various deletions of N-terminal sequences (as indicated) were transfected and processed as above. <b>E)</b> Representative images of the localization of the transfected mutant RanBPM quantified in D. Scale bar: 10μm.