ABCE1 suppresses RNAi of <i>GFP</i> in <i>C</i>. <i>elegans</i>.

<p><b>(A)</b> Schematic representation of the <i>in vivo</i> RNAi inhibition assay—the transgenes expressed and the GFP status are indicated. (<b>B)</b> Representative photomicrographs of the posterior part of animals expressing the NLS::GFP reporter in body wall muscles, treated with <i>GFP</i>-specific RNAi for 24 h at 20°C; control RNAi—empty vector. (<b>C)</b> Representative photomicrographs of the posterior part of animals expressing <i>C</i>. <i>elegans</i> ERI-1 and the NLS::GFP reporter in body wall muscles, treated with <i>GFP</i>-specific RNAi for 24 h at 20°C; ERI-1(+)—animals carrying the <i>myo-3</i>::<i>eri-1</i> transgene; ERI-1(-)—animals not carrying the <i>myo-3</i>::<i>eri-1</i> transgene. (<b>D)</b> Representative photomicrographs of the posterior part of animals expressing ABCE1 and the NLS::GFP reporter in body wall muscles, treated with <i>GFP</i>-specific RNAi for 24 h at 20°C. ABCE1(+)—animals carrying the <i>myo-3</i>::<i>ABCE1</i> transgene; ABCE1(-)—animals not carrying the <i>myo-3</i>::<i>ABCE1</i> transgene. (<b>E)</b> Relative GFP fluorescence intensity of the NLS::GFP reporter in worms expressing ERI-1 and ABCE1 and treated with <i>GFP</i>-specific RNAi for 24 h at 20°C. The ratio of the GFP signal intensity in worms expressing ERI-1 or ABCE1 and the NLS::GFP reporter compared to reporter alone is presented. Results from a representative experiment are shown (n > 100). Error bars represent the 95% confidence interval for the mean. Asterisks denote a statistically significant increase of the GFP signal intensity in worms expressing ERI-1 or ABCE1 and the NLS::GFP reporter as compared to the reporter alone (p < 0.0001 by two-tailed Student’s <i>t</i>-test), showing that ERI-1 and ABCE1 are able to suppress <i>GFP</i> RNAi.