Depleting RAD18 suppressed foci formation at the G2/M phase by DNA damage signaling factors in response to IR.

<p>HEK293 cells transfected with si-ctrl or si-RAD18 were exposed to 2 Gy IR, labeled with EdU, and then fixed at 90 min after irradiation. The cells were co-immunostained with anti-Edu and anti-γH2AX, anti-phospho-ATM or anti-53BP1 antibodies. The G1, S, G2/M phase cells were distinguished using the IN Cell Analyzer. The number of foci per cell was determined using the image-analysis software of the IN Cell Developer. Each value represents the mean (+standard deviation) of the results from three independent experiments. Each value represents the mean (+standard deviation) of the results from three independent experiments. (A) γH2AX, (B) phosphor-ATM, (C) 53BP1.