P2Y₂^-/- TL proliferate less and secrete less cytokines in response to in vitro IRBP restimulation.

<p>Twelve days after s.c. immunization with IRBP 1–20 peptide, P2Y₂^+/+ and P2Y₂^-/- mice were sacrificed and their spleen and draining lymph nodes collected and dissociated. (A) Splenic TL were semi-purified by passage on nylon wool fiber columns and pooled with LN cells before being in vitro restimulated in medium alone (CTRL) or supplemented with IRBP 1–20 peptide. TL proliferation was tested after 72h by (³H)- incorporation. Data are from a representative experiment of 10 with 4 mice per group. (B) Cytokine secretion was quantified, by specific ELISA, in culture supernatants after 48h of IRBP restimulation. Data are from a representative experiment of 5 or 6 or 3 respectively, with 4 mice per group. ns: not significant; **<i>p</i> < 0,01; ***<i>p</i> < 0,001.