Fluorescent microscopy images of Hoechst 33258-PI double staining.

<p>Two separate pictures from the same field were taken for the two markers. HeLa cells were treated with vehicle (control), or with <b>5f</b>, <b>5g</b>, <b>5h</b> and <b>4i</b> at the indicated concentrations. The blue fluorescence (left panels) indicates Hoechst 33258, and the red fluorescence (right panels) is a consequence of PI accumulation. The bar in the PI control picture indicates 100 μm.