Fluorescein and propidium iodine staining of hCTEpi cells treated with MPS.

<p>hTCEpi cultures were treated with hCTEpi growth medium containing 25% ReNu MultiPlus® MPS or PBS (control) and stained with Hoescht 33342, propidium iodide and fluorescein following an overnight incubation. Typical appearance of control cells is shown after Hoescht 33342 staining (A), propidium iodide staining (B) and fluorescein staining (C), with the equivalents for MPS-treated cells shown in (D) to (F) (in all cases n = 6 wells). The numbers of fluorescein hyperfluorescent cells clearly increased after MPS-treatment. Importantly hyperfluorecent cells were not also found to stain strongly with PI (one example is indicated by the circle). Data shown are representative of several experiments. Scale bars in (A) to (F) represent 100 µm.