Effect of curcumin and curcumin-PLGA conjugate on the clonogenic ability for cell survival.

<p>The colony formation assay was performed by crystal violet staining. HCT 116 cells were treated with 10 μM and 20 μM of curcumin (C) and curcumin-PLGA conjugate (CP) for 6 h, 12 h and 24 h. Similarly, 0.8 μl DMSO was used as a vehicle control. The cells were harvested, washed, and counted. 1000 cells were seeded in 6 well plate and allowed to grow for one week. At the end of incubation, cells were fixed with methanol and stained with 0.2% crystal violet stain. The cells were washed thrice to remove excess stain. (A) Representative image of colony formation. (B) The quantitative representation of colony formation. The plating efficiency was determined from a number of stained colonies. The graphs represent the plating efficiency of cells at mentioned time points and doses. Error bars represent mean ±SEM of three independent experiments. Significant difference indicated as *p≤0.05 between untreated and curcumin treated cells; ^#p≤0.05 between untreated and curcumin-PLGA conjugate treated cells; ^§p≤0.05 between curcumin and curcumin-PLGA conjugate treated cells (One way ANOVA followed by Student Newman-Keuls multiple comparisons test).