Impacts of curcumin on cellular ROS of RAW264.7 cells under oxidative stress.

M3 was drawn according to the left zone of the mode in negative control group, which represented the percentage of the cell in this zone. (a) RAW264.7 cells were cultured for 24 h. (b) RAW264.7 cells were cultured for 24 h, and 500 μM H2O2 was added 4 h before harvest. (c, d, e) RAW264.7 cells were cultured for 24 h with 5 μM, 10 μM or 20 μM curcumin. Then, 500 μM H2O2 was added 4 h before harvest. (f) RAW264.7 cells were cultured for 24 h, and 500 μM H2O2 was added 8 h before harvest. (g, h, i) RAW264.7 cells were cultured for 24 h with 5 μM, 10 μM or 20 μM curcumin. Then, 500 μM H2O2 was added 8 h before harvest. (j) A summary of the above results. The percent fluorescence intensity is expressed as the mean±SE of three independent experiments. *P **P P 2O2, ##P 2O2, ANOVA analyses.