Imaging spontaneous and evoked calcium activity of the same identified neurons during the awake and anesthetized state.

<p>(A) Image of detected (red and blue encircled) neurons and astrocytes in the awake mouse. Black traces (right) are the neuropil contamination-corrected ΔF/F calcium time series of the red-encircled neurons in the image (left). Vertical lines above the calcium time series indicate times when visual stimuli (gratings) were presented, each movement direction in a different color. The blue trace represents the evolution of the mean correlation between all pairs of displayed neurons (calculated over a sliding two-second window). The red trace shows animal movement, measured from treadmill motion. The green traces indicate the (corrected) horizontal (x) and vertical (y) image displacement, which was highly correlated with animal motion. Respective scale bars are located on the right, grey horizontal lines represent the horizontal zero axis. (B) Idem as in A, but recorded 45 to 70 minutes later under anesthesia (1.0% isoflurane in O2). Note the difference in scaling of the ΔF/F signals with (A).