IFNAR is required for VLP post-exposure protection against EBOV.

<p>(A) <i>Ifnar</i>^<i>-/-</i> and <i>Ifnar</i>^<i>+/+</i> (WT) mice (n = 10 mice/group) were infected i.p. with ~1000 PFU ma EBOV, followed 24 h later by injection i.p. with 50 μg of EBOV VLPs. One group of <i>Ifnar</i>^<i>+/+</i> (WT) mice infected with EBOV without VLP served as control. Mortality is expressed as percent survival of each group on indicated days. Results are a representative of three independent experiments, which gave very similar outcomes. QRT-PCR detection of EBOV GP mRNA level on day 2 post-EBOV infection with or without VLPs from liver (B) and spleen (C) of WT or <i>Ifnar</i>^<i>-/-</i> mice. GP transcripts were normalized by Hprt and values represent the mean ± SEM of duplicate samples from three independent experiments. Asterisk denotes significant differences compared to WT controls (*<i>p</i> ≤ 0.05, **<i>p</i> ≤ 0.01).