Late replication cycle events and cell surface virion assembly of ECTV are diminished in RK13 cells.

<p><b>(A)</b> Cells were grown on sterile glass coverslips, infected (MOI = 5) with ECTV, fixed 24 hours later, and then stained for the presence of cell surface B5 protein. Representative images are shown only for infected conditions because staining of uninfected control cells yielded no detectable signal. All images are at 400x total magnification. <b>(B)</b> Cells, which had been infected (MOI = 5) for 24 hours with ECTV, were stained for surface B5 and intracellular E3 following the protocol outlined in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0119189#sec002" target="_blank">Methods</a> section. All data shown are representative of three independent experiments.