Behavioural data of the differentially conditioned honey bees: hissing, escapes from odour and combination of these two.

<p>a) Hissing of bees during odour presentation (during section)—same data as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0118708#pone.0118708.g003" target="_blank">Fig. 3b</a>. b) Escapes by the bees from odour. Left panel: acquisition, right panel: recall test. Bees learned to escape from the odour during conditioning and escaped with a higher probability from the CS+ than from the CS- from the second trial on. This difference remained stable throughout the recall test. c) Proportion of bees that both hissed and escaped from the odour. Although only few bees exhibited both behaviours, there is a very clear difference between CS+ and CS-: almost no bees responded with hissing and escape to the CS- (2%), whereas 24.2% of the bees responded to the CS+ with both behaviours at the same time during the test. Solid lines and bars: CS+, dashed lines and shaded bars: CS-. Asterisks indicate statistically significant difference (RM-ANOVA followed by Fisher LSD-post hoc test, p<0.0001); error bars denote 95% confidence interval. d, e) Proportion of bees hissing, escaping, doing both or none of these responses upon presentation of CS+ (d) and CS- (e). The dashed line separates the conditioning phase (trial 1–4) from the recall test phase (trial 5 and 6).