Dose-dependent effects of cytostatic drug treatment on B16 cell proliferation and viability.

<p>Proliferation was determined by an MTT assay (mean ± SD, n = 4) for genistein (GEN, square), fingolimod (FIN, triangle) and betulin (BET, circle) in the B16 melanoma cell lines. B164A5 (A) and B16F10 (B) cells were treated at concentrations ranging from 1 to 150 μM for 24 h and stained with the MTT reagent for 4 h. Additionally, the percentage of living cells was tested by trypan blue exclusion (mean ± D, n = 5) for the B164A5 (C) and B16F10 (D) melanoma cell lines. The cells were treated as described above and stained with trypan blue after 24 h. Significant differences compared to the untreated control cells were calculated by two-way ANOVA with a Bonferroni post-test.