<p>(A, B, C) JHU-22<sup>vect</sup> and JHU-22<sup>miR-128</sup> protein levels in cell lines were evaluated by Western blotting analysis. The amount of protein was normalized relative to the intensity of ß-actin band and was semi-quantified based on relative intensity. (D Immunohistochemistry (IHC) effects of miR-128 expression in xenograft tissues. The levels of BMI-1, PCNA and cyclin D1 in the xenograft tissues were analyzed using IHC analysis. All IHC measurements were based on semi-quantitative scoring.</p
<p>(A) The protein levels of putative targets determined by Western blotting analysis. The amount of...
<p>(A) Growth curves of C2C12 myoblasts were determined by MTT assay. Cells were transfected with mi...
<p>A) q-RT-PCR of p27Kip1 mRNA levels in treated tumors retrieved from mice after intravenous LNA-i-...
<p>(A) SW480 and LoVo cells were transfected with <i>Pre-miR-124</i> or <i>Pre-Scrambled</i> miRNA a...
<p>(<b>A</b>) Representative gel of the RT-PCR amplification of <i>Nf1</i> mRNA in HEK293 cells tran...
<p>(A) The levels of cell growth-associated proteins in HCT-116<sup>vec</sup> and HCT-116<sup>miR-21...
<p>(A) qRT-PCR analysis of miR-127 expression in WI-38, IMR-90, MCF7, and MDA-MB-231. U6 RNA was use...
<p>Quantification of <b>A,</b> Ki67 (cell proliferation) and <b>B,</b> cleaved PARP (C-PARP; apoptos...
<p>U87 and U251 cells were differentially treated with SNAI1 shRNA and miR-128 ASO. (A), (B) and (C)...
<p>(<b>A</b>) The mRNA expression of Bcl6 and Setd8 were analyzed by qRT-PCR. β-actin was used as th...
<p>Cell growth was measured by an MTT assay. The experiment was repeated at least twice, each with t...
<p>(A) Western blot analysis of epithelial marker (E-cadherin) and mesenchymal markers (Vimentin, β-...
<p>Cells were exposed to MIR for 48 h, and harvested for RNA and protein extraction. (A) Gene expres...
<p>miR-221(A) q-RT-PCR 24, 48 and 72 hours after transfection with LNA-i-miR-221 and LNA-i-miR-NC in...
<p>U87 cells stably expressing miR-128 or miR-SCR were trypsinized and resuspended in serum-free med...
<p>(A) The protein levels of putative targets determined by Western blotting analysis. The amount of...
<p>(A) Growth curves of C2C12 myoblasts were determined by MTT assay. Cells were transfected with mi...
<p>A) q-RT-PCR of p27Kip1 mRNA levels in treated tumors retrieved from mice after intravenous LNA-i-...
<p>(A) SW480 and LoVo cells were transfected with <i>Pre-miR-124</i> or <i>Pre-Scrambled</i> miRNA a...
<p>(<b>A</b>) Representative gel of the RT-PCR amplification of <i>Nf1</i> mRNA in HEK293 cells tran...
<p>(A) The levels of cell growth-associated proteins in HCT-116<sup>vec</sup> and HCT-116<sup>miR-21...
<p>(A) qRT-PCR analysis of miR-127 expression in WI-38, IMR-90, MCF7, and MDA-MB-231. U6 RNA was use...
<p>Quantification of <b>A,</b> Ki67 (cell proliferation) and <b>B,</b> cleaved PARP (C-PARP; apoptos...
<p>U87 and U251 cells were differentially treated with SNAI1 shRNA and miR-128 ASO. (A), (B) and (C)...
<p>(<b>A</b>) The mRNA expression of Bcl6 and Setd8 were analyzed by qRT-PCR. β-actin was used as th...
<p>Cell growth was measured by an MTT assay. The experiment was repeated at least twice, each with t...
<p>(A) Western blot analysis of epithelial marker (E-cadherin) and mesenchymal markers (Vimentin, β-...
<p>Cells were exposed to MIR for 48 h, and harvested for RNA and protein extraction. (A) Gene expres...
<p>miR-221(A) q-RT-PCR 24, 48 and 72 hours after transfection with LNA-i-miR-221 and LNA-i-miR-NC in...
<p>U87 cells stably expressing miR-128 or miR-SCR were trypsinized and resuspended in serum-free med...
<p>(A) The protein levels of putative targets determined by Western blotting analysis. The amount of...
<p>(A) Growth curves of C2C12 myoblasts were determined by MTT assay. Cells were transfected with mi...
<p>A) q-RT-PCR of p27Kip1 mRNA levels in treated tumors retrieved from mice after intravenous LNA-i-...