MiR-103, miR-128 and miR-137 reduce endogenous NF1 protein but not mRNA expression.

<p>(<b>A</b>) Representative gel of the RT-PCR amplification of <i>Nf1</i> mRNA in HEK293 cells transfected with scramble or miR-128 vectors for 48 hours. The amount of starting template for each condition was equilibrated relative to <i>U6</i> RNA. Cycles were falling within the linear range of amplification for each primer pair. (<b>B</b>) Representative Western Blot analysis demonstrating that miR-128, miR-128/103, and miR-128/137 reduce endogenous NF1 protein levels in human cells. HEK293 cells were transfected with miR-128 constructs for 48 hours. 15 µg of whole-cell lysate was then loaded in each lane. B-tubulin was used as an internal control for loading. Data show the mean ± s.e.m from 4 (for RT-PCR) and 8 (for Western Blot) independent experiments (***, P<0.001). B