HMGB1 has no impact on cell surface expression of RAGE, TLR2 or TLR4.

<p>Eosinophils isolated from peripheral blood of normal donors incubated in the presence (open bars) or absence (closed bars) of IL-5 with 0, 10 or 100 ng/ml HMGB1for 48 hours and then evaluated for the expression of <b>(A)</b> RAGE, <b>(B)</b> TLR4, and <b>(C)</b> TLR2. Data represent mean ± SEM of percent population of live eosinophils that are positive for indicated receptor minus staining with isotype control; n = 3 independent donors.