Obesity induces a dramatic loss of Vγ9Vδ2 T cells.

<p>PBMC isolated from whole blood of obese and non-obese donors were analyzed by flow cytometry. (A) Gating strategy of peripheral T cell subsets used to examine CD3⁺, αβ⁺, and Vδ2⁺ T cells in non-obese and obese donors. (B) Percentage of total T cells (CD3⁺ cells) in NOD and OD. Each dot represents an individual donor and horizontal lines represent the mean. (C) Percentage of Vδ2⁺ T cells in NOD and OD. Median 2.715% NOD and 0.618% OD. (D) Correlation of body mass index (BMI) to Vδ2⁺ T cell percentages. (E) Number of γδ T cells per 1x10⁶ human PBMC in NOD and OD. Mean 18,576 NOD and 8,783 OD. (F) Percentage of αβ ⁺ T cells in NOD and OD. Median 96.2% NOD and 97.3% in OD. (G) Percentage of CD4⁺ and CD8⁺ αβ T cells in NOD and OD. P-values calculated from Pearson’s unpaired Student’s <i>t</i>-test.