Phylogenetic trees based on the complete genome, aa sequences of structural proteins and ORF3 of PEDV strains.

<p>The trees were constructed by the distance-based neighbor-joining algorithm using MEGA 5.2.2 software. Bootstrap was set in 1,000 replicates with a value >70% to assess the significance of the tree topology. A bar of 0.002/0.005 indicates nucleotide or amino acid substitutions per site. “●” indicates the strains identified in this study, “○” indicates the strains from China, “◆” indicates the strains from Belgium, “■”indicates the strains from USA, “▲”indicates the strains from South Korea, “▼”indicates the strains from France, “◇”indicates the strains from Japan. 1A: Phylogenetic tree generated on the basis of nucleotide sequences of the complete genome of 33 PEDVs. 1B to 1F: Phylogenetic trees based on deduced amino acid sequences of S glycoprotein genes, ORF3, envelope, membrane, and nucleocapsid genes, respectively.