Intensity scatter plots of FH with β-actin shows a colocalization in the HUVEC cytoplasm.

<p>HUVECs were formaldehyde-fixed, treated with Triton-X and stained concurrently for FH and <b>β</b>-actin. (A) FH was detected using 2 mouse monoclonal antibodies to human FH plus donkey anti-mouse AF IgG-488 (green). (B) <b>β</b>-actin was detected using polyclonal goat anti- <b>β</b>-actin plus chicken anti-goat antibody AF IgG-647 (red). (C) Shows the merged image detecting both FH (green) and <b>β</b>-actin (red) and the calculated values for the Pearson’s (PCC) and Manders’ (M1 and M2) correlation coefficients. (D) The intensity scatter plot of the merged image in (C) shows a single linear correlation indicative of a signal overlap.