Factor H is not present in HUVEC WPBs.

<p>Un-stimulated HUVECs were fixed with 1% p-formaldehyde and treated with 0.02% Triton-X to allow intracellular staining, and then stained with rabbit anti-human VWF antibody plus chicken anti-rabbit IgG-Alexa Fluor (AF) 488-secondary antibody. In panels A, B and C, VWF staining was followed by staining with two mouse anti-human FH monoclonal antibodies plus goat anti-mouse F(ab’)₂-IgG-AF 647-secondary antibody. Cells were imaged at 100X and cell nuclei were detected with DAPI (blue). (A) FH detection, red-647 channel; (B) VWF detection, green-488 channel and (C) merged image with simultaneous VWF and FH detection. In panels D, E and F, VWF staining was followed by FH detection using goat anti-human FH antibody plus donkey anti-goat IgG-AF 647-secondary antibody and imaging at 60X. (D) FH detection, red-647 channel; (E) VWF detection, green-488 channel; and (F) merged image with simultaneous VWF and FH detection. Images shown are representative of VWF and FH staining in >10 separate HUVEC cultures.