Protection effect on ϕPD10.3 and ϕPD23.1 on potato tuber slices (A) and whole tubers (B) against <i>D</i>. <i>solani</i> IPO2222, <i>P</i>. <i>carotovorum</i> subsp. <i>carotovorum</i> Ecc71 and <i>P</i>. <i>wasabiae</i> 3139.

<p>(A) Reduction of maceration ability of mixture of <i>D</i>. <i>solani</i> IPO2222, <i>P</i>. <i>carotovorum</i> subsp. <i>carotovorum</i> Ecc71 and <i>P</i>. <i>wasabiae</i> 3139 co-inoculated with bacteriophage ϕPD10.3 and ϕPD23.1 in potato tubers slices. The effect was determined by measuring the diameter of rotting tissue (in mm) after 72 h incubation at 28°C in a humid box. Wells of potato slices were filled up with 50 μl of sterile water (negative control), with 50 μl of bacterial suspension in water containing 10⁷ cfu ml^-1 of <i>D</i>. <i>solani</i> IPO2222, <i>P</i>. <i>carotovorum</i> subsp. <i>carotovorum</i> Ecc71 and <i>P</i>. <i>wasabiae</i> 3139 (positive control) or with 50 μl of bacterial suspension in water containing 10⁷ cfu ml^-1 of <i>D</i>. <i>solani</i> IPO2222, <i>P</i>. <i>carotovorum</i> subsp. <i>carotovorum</i> Ecc71 and <i>P</i>. <i>wasabiae</i> 3139 and 10⁵ pfu ml^-1 of ϕPD10.3 and/or ϕPD23.1 bacteriophage. Three potato slices containing 3 wells each and derived from three different tubers were used per experiment. The experiment was independently repeated one time and the results were averaged. (B) Reduction of infection ability of mixture of <i>D</i>. <i>solani</i> IPO2222, <i>P</i>. <i>carotovorum</i> subsp. <i>carotovorum</i> Ecc71 and <i>P</i>. <i>wasabiae</i> 3139 co-inoculated with bacteriophage ϕPD10.3 and ϕPD23.1 in the vascular tissue of potato tubers. The effect was determined by measuring the percentage of rotting tuber tissue (in g) after incubation the tubers at 28°C in a humid box. Caps of rose ends were removed from tubers with a sterile knife and resulting capless tubers were inoculated with 100 μl of bacterial suspension in water containing 10⁷ cfu ml^-1 of <i>D</i>. <i>solani</i> IPO2222, <i>P</i>. <i>carotovorum</i> subsp. <i>carotovorum</i> Ecc71 and <i>P</i>. <i>wasabiae</i> 3139 (positive control) or with 100 μl of bacterial suspension in water containing 10⁷ cfu ml^-1 of <i>D</i>. <i>solani</i> IPO2222, <i>P</i>. <i>carotovorum</i> subsp. <i>carotovorum</i> Ecc71 and <i>P</i>. <i>wasabiae</i> 3139 and co-inoculated with 100 μl of 10⁵ pfu ml^-1 of ϕPD10.3 and/or ϕPD23.1 bacteriophage. Ten potato tubers were used per treatment. The experiment was independently repeated one time and the results were averaged.