Strain RM1221 is unable to form a monospecies biofilm but exhibits both swarming and autoagglutination (AAG).

<p>Biofilm formation (A) of RM1221 (light grey bars) was measured by crystal violet staining and compared to NCTC 11168 (white bars), 81116 (dark grey bars), and a test tube containing only Brucella medium (black bar). Swarming ability (B) was calculated by measuring halo area on soft agar after 48 hours incubation in microaerobic conditions. Autoagglutination assessment (C) was carried out by observing the reduction in A₆₀₀ measurement over a 24 hour period. Both B and C show data for 11168 (white bars), RM1221 (light grey bars), 81116 (dark grey bars) and 11168 <i>ΔflaAB</i> (dark grey bars). Bars represent the median, error bars show range and significance was measured using Mann-Whitney tests (* = <i>P<0</i>.<i>05</i>).