Competition between serum IgG, IgA, and IgM anti-glycan antibodies.

<p>(A) Addition of IgM and IgA to IgG. Polyclonal IgG isolated from serum was first profiled on the array alone. Separately, IgG was premixed with varying amounts of IgM or IgA and then profiled on the array. For each array component, the change in IgG signal in the presence of IgM or IgA was determined. The box plots depict the range of IgG changes (on a log base 2 scale) measured on the array upon addition of 4 serum equivalents of IgM or IgA. The line in the middle of the box is the median, the box spans 1 standard deviation above and below the median, and the whiskers represent 2 standard deviations above or below the median. (B) Addition of IgG and IgA to IgM. An analogous protocol as above was used to evaluate effects of IgG and IgA on IgM signals. (C) Addition of IgM and IgG to IgA. An analogous protocol as above was used to evaluate effects of IgG and IgM on IgA signals. The box plots demonstrate significant decreases in IgG and IgA signals in the presence of IgM for the vast majority of array components.