Three-step vs jacalin affinity purification of mucosal immunoglobulins.

<p>Box plot comparing IgA and IgG immunoglobulin purification by two different methods assayed in the study. Left panel shows IgA and IgG values measured in jacalin-bound and -excluded fractions, respectively; right panel presents IgA and IgG values measured in the two bound fractions. Values are expressed in total micrograms per sample, in log-scale. From the left to the right, respectively: Jacalin–agarose purification (white box): IgA from healthy controls; IgA from HIV-positive subjects; IgG from healthy controls; IgG from HIV-positive subjects. Three-step affinity purification (grey box): IgA from healthy controls; IgA from HIV-positive subjects; IgG from healthy controls; IgG from HIV-positive subjects. Box legends: Box short sides represent the third (Q3) and the first quartile (Q1), respectively, while the horizontal bar (-) shows the median value. Vertical lines above or below the box indicate the corresponding quartile value (Q3 or Q1) plus or minus 1.5 times the interquartile interval (IQ = Q3−Q1).