Epistasis analysis of <i>elt-1(ku491); daf-12(rh61rh411)</i> mutant phenotypes with RNAi of other heterochronic genes.

<p>^aL4 Bursting Vulva, Alae Formation, and Young Adult Seam cell phenotypes were analyzed for strains of indicated genotypes and RNAi treatment. <i>elt-1(rf)</i> is the partial loss-of-function allele <i>ku491</i>. <i>daf-12(lf)</i> is the loss-of-function allele <i>rh61rh411</i>.</p><p>^bFor bursting vulva rate, the p-value for the comparison of <i>elt-1(ku491); daf-12(lf)</i> animals on empty-vector (-) RNAi vs target gene RNAi is < 0.0001 for each of <i>lin-28</i>, <i>hbl-1</i>, <i>lin-14</i>, <i>lin-41 and mab-10</i>, 0.003 for <i>lin-46</i>, 0.0002 for <i>lin-42</i>, 0.983 for <i>kin-20</i>, 0.0003 for <i>ceh-16</i>, and 0.315 for <i>dre-1</i>.</p><p>^cFor seam cell numbers, the p-value for the comparison of <i>elt-1(ku491);daf-12(lf)</i> animals on empty-vector (-) RNAi vs on target gene RNAi is <0.0001 for <i>lin-28</i>, <i>hbl-1</i>, <i>lin-14</i>, <i>lin-41</i>, <i>lin-46</i>, <i>lin-42</i>, <i>kin-20</i>, <i>mab-10</i>, <i>ceh-16</i>, and <i>dre-1</i>, and > 0.9999 for <i>lin-29</i>.</p><p>Abbreviations: e.v., empty vector; Abs, absent; Gap, gapped; Pres, present; #, seam cell number measured using the <i>scm</i>::<i>GFP</i> reporter; Std Dev, standard deviation.</p><p>Epistasis analysis of <i>elt-1(ku491); daf-12(rh61rh411)</i> mutant phenotypes with RNAi of other heterochronic genes.