C4G2A interaction with sg2AtSKs <i>in planta</i> is localized to the cytosol and nucleus.

<p>A. Comparative strength of the interactions between C4 protein and C4G2A interactions with AtSK23. ß-galactosidase activity was quantified using triplicate samples from three individual yeast colonies and is expressed relative to the C4/AtSK23 positive control interaction. The interaction between C4 and Lamin C is included as a negative control. B. Confocal micrographs of the interaction between mutant C4G2A-cEYFP with AtSK23-nEYFP following co-agroinfiltration into CFP-H2B marker plants. From left to right, the first column shows the interaction assay (EYFP), the second column shows the nuclear signal from the reference marker (CFP-H2B), the third shows the merger between the two preceding panels (Overlay) and the fourth an enlargement of the boxed region in the Overlay. The co-localized EYFP signal appears in yellow, nuclear reference CFP fluorescence appears in blue, and the chloroplast autofluorescence is shown in red. C. Confocal micrograph of C4G2A fused to EYFP following agroinfiltration into transgenic <i>N</i>. <i>benthamiana</i> CFP-H2B marker plants. Arrowhead, chloroplasts; arrows, cytoplasmic strands. Scale bar = 50 μm.