Subcellular localization of C4 protein.

<p>A) The fluorescence observation of control GFP protein in Arabidopsis protoplasts. Bar = 10 µm. (B) The fluorescence observation of GFP-C4 fusion protein in Arabidopsis protoplasts. Bar is common to (A). (C) The fluorescence observation of control GFP protein in <i>N. benthamiana</i> leaves. Bar = 20 µm. (D) The fluorescence observation of GFP-C4 fusion protein in <i>N. benthamiana</i> leaves. Bar is common to (C). Cells were analyzed by confocol microscopy. F indicates fluorescence, B indicates bright light and M indicates merged. (E) Cell fraction assays of GFP and GFP-C4 fusion protein. Total extract of <i>N. benthamiana</i> leaf cells expressing a GFP control and GFP-C4 fusion proteins were fractionated into soluble (S) and microsomal (M) fractions. GFP and GFP-C4 fusion proteins were detected using a anti-GFP antibody and indicated (top panel). Ponceau S staining of the transferred membrane is displayed as a loading control (bottom panel).