BMAL1 and PER1 protein and transcript levels are reduced by thyroxine treatment.

<p>HFs treated with T4 for 24 hours were stained for either BMAL1 or PER1 and transcript levels were assessed. (a) qRT-PCR demonstrated that CLOCK, BMAL1 and PER1, were significantly down regulated by T4 expression (24 hours). Protein expression was also assessed and quantified using immunohistomorphometry. (b,d) PER1 protein was significantly decreased by T4 at 6, 12,18 and 24 hours. BMAL1 protein levels were also decreased significantly by T4 at time points 6,12 and 18 hours showing a tendency to decrease at 0 and 24 hours. (mean (SD) * p < 0.05, *** p < 0.001, Student’s Ttest mean, results were pooled from multiple HFs from 3 patients). (scale bar = 50μm)