CXCL8 signaling regulates the expression of thymidylate synthase (TS) in PC3 cells.

<p>(<b>A</b>) Bar graph presenting the time-dependent change in TS transcript levels in PC3 cells (left panel) and LNCaP cells (right panel) as detected by qPCR analysis following treatment with 3 nM rhCXCL8. Expression is presented as a fold-change relative to gene expression levels detected in untreated cells. (<b>B</b>) Immunoblot demonstrating the effect of administering 3 nM rhCXCL8 upon the level of TS expression in PC3 cells, detected up to 1 h post-stimulation of the cells. (<b>C</b>) Immunoblot that illustrates the level of TS expression detected in PC3 cells (left panel) and LNCaP cells (right panel) up to 8 h post-stimulation of the cells with rhCXCL8. Equal protein loading in each of the representative blots shown in (B) and (C) was confirmed by re-probing of the membranes to detect expression of GAPDH. Statistically significant differences were determined using a Students two-tailed t-test (***, p<0.001).