CAPER inhibition reprograms nuclear transcriptomes and metabolomes resembling retrograde responses.

<p>A. Pie charts showing the IPA results of top physiological functions (i) and molecular functions (ii) enriched in CAPER-dependent transcriptomes. Each section represents the percentage of the genes in the indicated functional category out of entire CAPER-dependent transcriptomes. B. A graph showing the IPA results of top canonical pathways enriched in CAPER-dependent transcriptomes. Each bar represents log₁₀ (p value) of each functional category out of the entire CAPER-dependent transcriptomes. C. Depletion of CAPER disturbs cellular carbon and nitrogen balance. A heat map (left panel) showing altered metabolites by Log ₂ (FC: fold change) (left colum-24 hour samples) and by Log ₁₀(p values) (p values adjusted for multiple comparisons using Benjamini-Hochberg method) (right column). Scale of red (positive values) and green (negative values) are represented as a color code on top of the figure. A matrix (right panel) showing the correlation distance calculated by Spearman’s correlation analysis among the metabolites from cells 24 hours after transfection with siControl and siCAPER. Scale of red (positive values) and green (negative values) are represented as a color key on top of the figure.