Baclofen reverses altered expression of GABA_BRs in the lung tissue from rats subjected to IC induced ALI.

<p>(A) Lung tissue homogenates from three animal groups: control (lane 1), lung injury (LI) (lanes 2 and 3), or LI followed by baclofen administration (LI+B) (lanes 4, 5, and 6), were subjected to immunoblot analysis with anti-GABA_BR2 and anti-GAPDH antisera. (B) GABA_BR2 and GAPDH immunoblots from above three animal groups were scanned and band densities were quantified using ImageJ software GABA_BR2 band density is normalized to its respective GAPDH band intensity. Data is expressed as mean ± SEM (n = 3 animals per group). (C) Lung tissue homogenates from five animal groups: control, lung injury (LI), or LI followed by baclofen administration (LI+B), CGP52432 pretreatment followed by lung injury (CGP+LI), and CGP52432 pretreatment followed by lung injury and baclofen administration (CGP+LI+B) were subjected to immunoblot analysis with anti-GABA_BR2 and anti-GAPDH antisera. (D) GABA_BR2 and GAPDH immunoblots from above five animal groups were scanned and band densities were quantified using ImageJ software GABA_BR2 band density is normalized to its respective GAPDH band intensity. Data is expressed as mean ± SEM (n = 3 animals per group).