Prevention of IC deposition-induced lung apoptosis by administration of baclofen.

<p>(A) Lung tissue sections from three animal groups: control, lung injury (LI), or LI followed by baclofen administration (LI+B), were subjected to terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining to study the changes in apoptosis in various groups. Shown are representative images with 20X images and zoomed images are shown below. N = 3 animals per group were subjected to TUNEL staining. (B) Lung tissue homogenates from three animal groups: control (lane 1), lung injury (LI) (lanes 2 and 3), or LI followed by baclofen administration (LI+B) (lanes 4 and 5), were subjected to immunoblot analysis with anti-cleaved caspase-3 and anti-GAPDH antisera. A representative blot is shown from n = 3 independent experiments performed with 3 animals per group. (C) Cleaved caspase-3 and GAPDH immunoblots were scanned and band densities were quantified using ImageJ software. Cleaved caspase-3 band density is normalized to its respective GAPDH band intensity. Data is expressed as mean ± SEM (n = 3 animals per group).