<p>DNA constructs were tested for expression by (A) SDS-PAGE electrophoresis and (B) Western blot analyses. Proteins were purified under reducing conditions and detected using an anti-His mAb.</p
<p><i>P. yoelii</i> (A) and <i>P. falciparum</i> (B) DNA vaccine candidates and control constructs w...
Western blotting is a standard laboratory method that uses antibodies to detect target proteins in a...
<p>Protein samples were either boiled at 95°C for 5 min, or not boiled, followed by addition of 6× S...
<p>Representative western blot of basal level expression for various molecules was performed. Briefl...
<p>The detection antibodies were anti-S (A) or anti-Pre-S monoclonal antibodies at a concentration o...
<p>The soluble protein of CC-2654 (2) were used as control, the soluble protein of transformants MT-...
<p>Conditioned media from HEK 293 cells transiently transfected with the bovine <i>SOLD1</i> gene we...
<p>Expression of His6-tagged EtLPP1, -2, -3 or pcDNA3 in 293 cells was separated by SDS-PAGE (12%) a...
<p>Supernatants of three different cell pools were purified using protein-A. Purified samples were a...
<p>(A) Western blot analysis of a panel of proteins was conducted on siNC- or siJAM-A-transfected H1...
<p>(A) Lsa66, (B) Lp30 and (C) rLIC12238 expression from NaCl-induced <i>E. coli</i> Bl21-SI. M: mol...
All antibodies mentioned were used for Western blot analysis as well as for immunhistochemistry as p...
<p>BHK-21 cells were mock-infected or infected with r-HN or the transfected supernatants at a MOI of...
<p>HEK 293T/17 cells were transfected with D2ET or D2E12 and cell lysates were collected on day 2 po...
A B S T R A C TWestern blotting is an important technique used in cell and molecularbiology. Using t...
<p><i>P. yoelii</i> (A) and <i>P. falciparum</i> (B) DNA vaccine candidates and control constructs w...
Western blotting is a standard laboratory method that uses antibodies to detect target proteins in a...
<p>Protein samples were either boiled at 95°C for 5 min, or not boiled, followed by addition of 6× S...
<p>Representative western blot of basal level expression for various molecules was performed. Briefl...
<p>The detection antibodies were anti-S (A) or anti-Pre-S monoclonal antibodies at a concentration o...
<p>The soluble protein of CC-2654 (2) were used as control, the soluble protein of transformants MT-...
<p>Conditioned media from HEK 293 cells transiently transfected with the bovine <i>SOLD1</i> gene we...
<p>Expression of His6-tagged EtLPP1, -2, -3 or pcDNA3 in 293 cells was separated by SDS-PAGE (12%) a...
<p>Supernatants of three different cell pools were purified using protein-A. Purified samples were a...
<p>(A) Western blot analysis of a panel of proteins was conducted on siNC- or siJAM-A-transfected H1...
<p>(A) Lsa66, (B) Lp30 and (C) rLIC12238 expression from NaCl-induced <i>E. coli</i> Bl21-SI. M: mol...
All antibodies mentioned were used for Western blot analysis as well as for immunhistochemistry as p...
<p>BHK-21 cells were mock-infected or infected with r-HN or the transfected supernatants at a MOI of...
<p>HEK 293T/17 cells were transfected with D2ET or D2E12 and cell lysates were collected on day 2 po...
A B S T R A C TWestern blotting is an important technique used in cell and molecularbiology. Using t...
<p><i>P. yoelii</i> (A) and <i>P. falciparum</i> (B) DNA vaccine candidates and control constructs w...
Western blotting is a standard laboratory method that uses antibodies to detect target proteins in a...
<p>Protein samples were either boiled at 95°C for 5 min, or not boiled, followed by addition of 6× S...