Bioenergetic profile of platelets exposed to thrombin.

<p>(A) OCR and (B) ECAR were measured in platelets by establishing a basal rate then injecting thrombin (0.5 U/ml) and following over 64 min. (C) either media or thrombin were injected (0.5 U/ml) and then sequential injections of 1 μg/ml oligomycin (O), 0.6 μM FCCP (F) and 10 μM Antimycin A (AA). (D) Different parameters of mitochondrial function were calculated—basal (basal OCR—AA sensitive OCR), thrombin (thrombin response—basal OCR), ATP-linked (thrombin response—oligomycin sensitive OCR), proton leak (oligomycin sensitive—AA sensitive OCR), maximal (FCCP sensitive—AA sensitive OCR), reserve capacity (FCCP sensitive—thrombin responsive OCR) and non-mitochondrial (AA sensitive OCR). (E) Simultaneously ECAR measured by first establishing a basal rate followed by injection of thrombin (0.5 U/ml). All the bioenergetic measurements were normalized to protein content per well. Data expressed as mean±SEM from one representative donor, n = 3–5 replicates per sample. *p<0.01, different from control.