Effect of inhibiting mitochondrial fatty acid oxidation on thrombin stimulated platelets.

<p>Platelets were plated on Cell-Tak coated XF96 plates, and pre-treated with etomoxir (25 μM) for 1h prior to bioenergetic measurements. (A) Basal OCR of platelets were measured prior to injection of thrombin (0.5 U/ml), followed by 1 μg/ml oligomycin (O), 0.6 μM FCCP (F) and 10 μM antimycin A (AA). (B) Indices of mitochondrial function, basal, thrombin responsive, ATP-linked, proton leak, maximal, reserve capacity and non-mitochondrial OCR were calculated. (C) Basal and thrombin responsive ECAR were calculated from parallel ECAR measurements. Platelets were pre-treated with TMZ (0–1000 μM) for 3h before bioenergetic assay. (D) Change in OCR after thrombin injection presented as a percentage of control. Data expressed as mean±SEM from one representative donor, n = 3–5 replicates per sample. *p<0.01, different from control. #p<0.01, different from thrombin. $p<0.01 different from etomoxir.