Inhibition of miR-20a slightly increases TCR-mediated signaling.

<p>Human naïve CD4⁺ T cells were transfected with miR-20a or scramble decoys. <b>(A)</b> Quantification of miR-20a expression in resting and in stimulated cells upon stimulation with plate-bound CD3 and CD28 mAbs for 45 min was performed using RT qPCR. <b>(B)</b> 16 hours after transfection, cells were stimulated with microbeads coated with CD3 and CD28 mAbs for the indicated time periods. Subsequently, lysates were analyzed by immunoblotting using the indicated Abs. Bands in <b>(B)</b> were quantified using the ImageQuant software and values were normalized to the corresponding β-actin signal. Graphs in <b>(C)</b> show the phosphorylation levels of the indicated molecules as arbitrary units ± SEM of 3 independent experiments. Significat P values were calculated by using Student’s t Test. (*, P < 0.05).