miR-20a regulates cytokine production.

<p>Human naïve CD4⁺ T cells were transfected with plasmids encoding either miR-20a or miR-control (miR-Ctrl). 16 h after transfection, cells were stimulated with plate-bound CD3 and CD28. 48 h after stimulation, supernatants were collected and cytokines were measured by ELISA. Graphs in <b>(A)</b> show relative cytokine levels from miR-20a overexpressing cells compared to miR-control. Graphs in <b>(B)</b> show the absolute values of cytokine concentration, in pg/mL, from individual experiments. Data expressed as arbitrary units ± SEM of at least 4 independent experiments. Significat <i>P</i> values were done by using Student’s <i>t</i> Test. (*, <i>P</i> < 0.05; **, <i>P</i> < 0.01; ***, <i>P</i> < 0.001).