The mRNA and protein expression of IL-6 and IL-8 in H4 cells.

<p>Gene expression was determined by qRT-PCR (A) and protein level was measured by ELISA (B). Expression levels of genes are normalized to glyceraldehyde-3-phosphate dehydrogenase (GADPH). Relative mRNA levels were calculated using the 2^-ΔΔCt method and the average ΔCt values of the unstimulated control group served as the calibrator. The gene expression and cytokine production in probiotic-conditioned media treatments was compared to the corresponding control group, unstimulated and IL-1β-stimulated, respectively. All data represent the mean ± the SEM (n = 3 for qRT-PCR and n = 4 for ELISA). A p<0.05 (*) or p<0.001 (**) depicts the significance value. BCM, <i>Bifidobacterium infantis</i>-conditioned media; LCM, <i>Lactobacillus acidophilus</i>-conditioned media.