Effects of LL-37 on plaque assays and neuraminidase inhibition with Phil82 IAV.

<p>Panel A compares the ability of LL-37 and GI-20 to inhibit plaque formation by Phil82 IAV. The virus was either pre-incubated with the peptides prior to the assay, or the peptides were added to the monolayers after allowing 45 min for virus to infect the cells (“Delayed Addition”). Panel B shows neuraminidase inhibition assay results using LL-37, sLL-37, or LL-37-related peptides. Phil82 virus was pre-incubated with the peptides and then NA activity was assayed as described in Materials and Methods. Oseltamivir was used as a positive control and it caused marked reduction of NA activity. Results are mean±SEM of 4 or more experiments. * indicates p<0.05 vs control buffer alone (unpaired t test).