Expression of the <i>bdv-p</i> but not <i>bdv-x</i> gene in hNPCs impairs neuronal differentiation.

<p>Transduced hNPCs expressing <i>gfp</i>, <i>bdv-p</i> or <i>bdv-x</i> genes and their matched NT controls were induced to differentiate for 14 days. (A) Immunostaining with antibodies directed against βIII-Tubulin, a neuronal marker (red), or GFAP, an astrocytic marker (green). Nuclei were stained with DAPI (blue). For panel homogenization, GFAP immunostaining performed in <i>gfp</i>-expressing hNPCs was re-colored in green. The percentage of neurons and astrocytes was determined based on enumeration of βIII-Tubulin-, GFAP- and DAPI-positive cells in (B) <i>gfp</i>-expressing hNPCs, (C) <i>bdv-p</i>-expressing hNPCs and (D) <i>bdv-x</i>-expressing hNPCs. Results in B, C and D are representative of 2, 5 and 2 independent experiments, respectively. All experiments were performed in triplicate. Statistical analyses were performed using the Mann-Whitney test. ***, p < 0.001, ns, non-significant (p > 0.5). N, neurons. As, astrocytes. Scale bar, 50 μm.