Proportion of <i>Wolbachia</i>-infected cells detected by Fluorescence <i>in situ</i> Hybridization.

<p>Rhodamine-labelled oligonucleotide probe designed on <i>Wolbachia</i> 16S rRNA gene (red) detected the bacteria in the cytoplasm of the host cell at passages P.15 and P.37 (<b>A</b>). Nuclei of the host cells are shown in blue after DAPI labelling (bars = 20μm). Percentage of cells with a <i>Wolbachia</i>-positive signal in FISH at P.15 and P.37 (<b>B</b>) (Error bars represent the standard deviation of the mean of 50 independent microscope fields from three independent samples).