Inhibition of wild type alphaviruses by Bortezomib.

<p>U87MG cells were pre-treated with Bortezomib at 0.1μM for 2 hours and then infected with VEEV TrD, EEEV strain GA97, and WEEV California 1930 strain at an MOI: 0.1 for 1 hour. Supernatants were collected 24 hours post infection and analyzed by plaque assay for infectious viral particles (PFU/mL). The arithmetic means are illustrated graphically. The graph is representative of 3 independent experiments where each experiment was performed in triplicate. Standard deviations were calculated accordingly. p<0.05(*).