Effects of siRNA TMPRSS2-ERG on ERG protein expression and cell viability.

<p>VCaP cells were transfected for 24h, 48h and 72h with 50 nM of siRNAs TMPRSS2-ERG III (1, 2, 3), siRNAs TMPRSS2-ERG IV (1, 2), siRNA against ERG wild type or siRNA Control. <b>a. Western blot analysis:</b> the expression of ERG protein detected by Western blot showed the effects of siRNAs against TMPRSS2-ERG, ERG wild type or siRNA Control. GAPDH was used as internal control (images are representative of three independent experiments). <b>b. MTT cell viability assay:</b> VCaP cells were transfected with siRNA TMPRSS2-ERG III (1, 2), TMPRSS2-ERG IV (1) and siRNA Control at 50 nM concentration and incubated for 72h. Bars represent mean ± SD of 2 independent experiments containing 8 replicates for each condition. The number of viable cells was measured and 100% cell viability corresponds to the number of living cells incubated only with transfecting agent. *** = <i>p</i><0.001, significant decrease compared to untreated cells or to siRNA Control (Kruskal & Wallis followed by Tukey and Dunnet test).