Effects of siRNA knockdown of <i>PPP2R4</i>.

<p>(a) Real-time polymerase chain reaction and western blot analysis of cancer cells transfected with <i>PPP2R4</i>-specific siRNA and control siRNA. (b) WST-8 assay using cancer cell lines transfected with the <i>PPP2R4</i> siRNA and control siRNA. (c) Caspase 3/7 activity in the cancer cell lines transfected with the <i>PPP2R4</i> siRNA and control siRNA. (d) Western blot analysis of cell signaling changes in endometrial cancer cells transfected with the PPP2R4 siRNA and control siRNA. The expression levels of phospho-extracellular signal-regulated kinase 1/2 (ERK1/2), ERK1/2, phospho-protein kinase B (AKT), AKT, phosphor-ribosomal protein S6 (rpS6), rpS6, phospho-glycogen synthase kinase-3 beta (GSK3β), GSK3β, and cyclin D1 in cancer cells transfected with the siRNAs are shown. Columns and error bars represent the mean ± the standard error of the mean for at least three independent experiments.