TGF- receptor inhibitors augment the effects of TKIs on colony growth formation.

<p>Clonogenic assays were performed where cells were treated with DMSO, 0.3μM AZD8010/AZD4547, 0.1μM gefitinib, or both in the presence or absence of a TGF- receptor I (TGFRI) small molecule inhibitor: SB525334 (1μM). After 2 weeks of incubation, colony growth was measured. This assay was performed in a panel of cell lines and is shown here for <b>A.</b> UMSCC25 <b>B.</b> 584-A2 <b>C.</b> UMSCC8 and <b>D.</b> HN31. <b>E.</b> Representative images of the experiment performed with UMSCC8 cells in C. *denotes a p-value <0.05; **denotes a p-value <0.006; *** and ****denotes a p-value <0.0001.