Detection of Lv-Pro-miRNA126 expression over time.

<p>(A, B) RAECs and ARAECs were infected with Lv-Pro-miRNA126, cultured for indicated times and miRNA126 RNA, VEGF and BCL-2 protein levels were assessed by quantitative PCR and western blotting, respectively. (C, D) Uninfected RAECs and ARAECs or ARAECs infected with Lv-Pro-miRNA126 or Lv-shRNA-BCL-2 were treated with or without OX-LDL for 48 hours and subject to an apoptotic assay. Representative plots are shown. Data are the means ± SD of at least 3 separate experiments. **, p<0.01, when compared to the RAECs group or the indicated group.