Loss of REV3 is synthetic lethal with ATRi and cisplatin.

<p>(A-F) H157 NSCLC cells were transfected with non-targeting siRNA (siNT) or two siRNAs targeting REV3 (number 2 and 4 refer to specific sequences described in the materials and methods). Cells were then treated with ATRi, cisplatin, and ATRi and cisplatin. Cell viability was determined with alamar blue and reported as a percent of the untreated control cells. (A) Sensitivity of REV3 knockdown cells to cisplatin. (B and C) Sensitivity of REV3 knockdown cells to ATRi and ATRi with 0.1μM cisplatin. Bliss independence synergy between ATRi and cisplatin in control (D) and REV3 knockdown cells (E and F). Error bars in all panels are standard deviation (n = 3).