Specificity of siRNA RET/PTC3 for inhibition of RET/PTC3 fusion oncogene expression in RP3 (I) and in BHP10-3 SCmice (II) cells.

<p>siRNAs (RET/PTC3, RET/PTC1 or Control) were transfected at 50 nM concentration in RP3 (<b>I</b>) and BHP10-3 cells (<b>II</b>). After 24 h, 48 h and 72 h post-transfection, RT-qPCR (<b>IA, IIA</b>) and Western blot (<b>IC, IIC</b>) were performed for relative mRNA and protein expressions. Quantification of relative RET protein expression (IB, IIB) was performed by Bio-Rad Image Lab software scanning, results are expressed as relative RET protein expression compared to non-treated cells. Data were analysed by one-way ANOVA followed by LSD Post-hoc test. Stars represent the significant difference between the treatment groups compared to non-treated cells. *** = <i>p</i><0.001.